A developmental study of Plants (Arabidopsis thaliana):

❖ Cloning of seed-specific transcription factors.

❖ Transformation into Arabidopsis by floral dip method.

❖ Analysis root, shoot, seed, and sliques specific phenotype.

❖ Establish the relationship between interacting partner to fish out the candidate gene for further study.

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Molecular genetic study on abiotic stress tolerance in rice (Oryza Sativa L.):

❖ Cloning of transcription factors into a binary vector.

❖ Agrobacterium-mediated transformation into rice callus.

❖ Transformation into green algae Chlorella vulgaris via electroporation, PEG and Agrobacterium methods.

❖ Confocal images analysis of chlorella protoplast.

❖ SEM analysis of rice pollen and anthers.

❖ Stress physiology analysis.

❖ Agronomical analysis

❖ Bioinformatics tools.

 

Identification and pyramiding of genes for improving salt tolerance and seed yield in Indian mustard (Brassica juncea L.):

❖ Extraction of genomic DNA from Brassica juncea and rice mutant by the cTAB method.

❖ PCR based amplification of SSR markers.

❖ Native PAGE of the amplified product.

❖ Silver staining of PAGE to visualize polymorphic bands.

❖ Dissimilarities analysis of Brassica juncea and rice mutants.

 

Development and evaluation of salt and drought tolerant transgenic rice:

❖ Agrobacterium-mediated transformation in the rice callus.

❖ Learning basic molecular biology techniques.

 

A green methodology to prepare commercially important products:

❖ To check the activity of esterase on β-ketoester +alcohol.

❖ Purification of a product by column chromatography.

❖ Nuclear magnetic resonance as well as mass spectroscopy of the product.

 

 

Technical Expertise:

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Molecular Biology:

Isolation of genomic/ plasmid DNA, Restriction digestion, Protein isolation, Western Blotting, Agarose & PAGE electrophoresis, cloning, Silver Staining, Southern blotting, Real-time PCR, IPCR, and cloning.

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Transformation:

Agrobacterium-mediated transformation into rice callus, floral dip into Arabidopsis and Chlorella vulgaris, electroporation, particle bombardment and PEG method transformations in to Chlorella vulgaris.

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Plant Tissue Culture:

Agrobacterium-mediated transformation of many TF genes in rice callus, floral dip method, sub-culturing, regeneration of rice callus (Indica cultivar IR-64, CSR-10, PB-1, and Japonica cultivar Dongjin, kittake).

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Plant Physiology:

Determination of Physiological parameters (Chlorophyll content, proline content, electrolyte leakage, Na/K ratio, etc.) in transgenic lines as well as mutant lines of rice. Cold, drought and salt stress analysis at seedling stage and at a mature stage, agronomical trait analysis, seeds germination test etc.

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Bio-organic Chemistry:

Chromatographic techniques (TLC, Column), Rotary Evaporator.

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Imaging techniques:

Confocal of Chlorella protoplast and SEM analysis of Rice pollen grains and anthers, compound microscopy, GEL doc., and hand camera photography for phenotype analysis.